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液相色谱-质谱分析法
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Multiple dose pharmacokinetics of artemisinin in rats by high performance liquid chromatography-tandem mass spectrometry
关键词:时间依赖性 药代动力学 青蒿素 液相色谱-质谱分析法
Aim To investigate the pharmacokinetics of the antimalarial artemisinin in rats after single and multiple oral doses. Methods Six male Wistar rats were orally given a single dose of artemisinin 40 mg·kg-1(Group 1). Another six rats were orally given artemisinin at the same dose once daily for 5 consecutive days(Group 2). Artemisinin plasma concentrations,after single dose or after 5 consecutive doses,were determined by high performance liquid chromatography-tandem mass spectrometry(LC/MS/MS). Results The artemisinin pharmacokinetic parameters of Group 1 were compared with that of Group 2. The areas under the plasma concentration-time curve(AUC0-8)decreased from 126.53 h·ng·mL-1 of Group 1 to 39.91 h·ng·mL-1 of Group 2(p0.05). Conclusions The optimized LC/MS/MS method was successfully applied to the pharmacokinetic study of artemisinin in rats after single and multiple oral doses. The results suggested that artemisinin exhibits time-dependent pharmacokinetics.
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